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Fig. 3 | Basic and Clinical Andrology

Fig. 3

From: Target antigens for Hs-14 monoclonal antibody and their various expression in normozoospermic and asthenozoospermic men

Fig. 3

2D electrophoresis in RHB of human sperm extract and immunoblot with Hs-14 mAb (a). Isoelectric focusing was carried out in pH 3–10, and the separation in the second dimension was run in 12 % polyacrylamide gel. On the corresponding blot the Hs-14-reacting proteins are indicated by arrows and were subjected to mass spectrometry. In some cases the Hs-14 mAb reacted on WB also with proteins of about 240 and 110 kDa, but these proteins had no counterparts in 2D gels. Therefore, the 240 kDa band for MS was selected from SDS PAGE according to the corresponding immunoblot (b)

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Basic and Clinical Andrology

ISSN: 2051-4190